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Fig. 4 Rescue of the MO-induced gitrl morphant phenotype by co-injection of synthetic capped gitrl mRNA. Co-injection of synthetic capped mRNA and rescue of a normal phenotype was used to confirm that the MO-induced gitrl phenotype was specific and not due to off-target effects. Results with zGL1 (translation block) are shown, although equivalent rescue was also obtained for zGL2 (splice-block). The data are representative of embryos from at least three independent experiments in which a minimum of thirty embryos per MO per experiment were injected.
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Full text @ Int. J. Dev. Biol.