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Fig. S1 RT-PCR analysis of embryos injected with a katna1 splice-blocking morpholino (katna1e4i4). Embryos were injected with 0.9 pmol or 1.8 pmol of katna1e4i4 or control morpholino (CoMO). RT-PCR with primers spanning exons 2-6 gave a 646 bp product in CoMO-injected embryos and an additional smaller product consistent with skipping of exon 4 (646-187=459 bp) in katna1e4i4-injected embryos. The increased severity of motor axon outgrowth defects in embryos injected with the higher dose of the morpholino correlated with increased levels of the shorter product.
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Full text @ Dis. Model. Mech.