Image
|
Figure Caption
Fig. S2 Confirmation of altered splice site use in tnnc1a, tnnc1b, and tnnc2 morpholino-injected embryos. mRNA was isolated from pooled embryos at 1 dpf and analyzed using primers flanking the morpholino-targeted exon donor site. PCR was performed using the Qiagen OneStep RT-PCR kit. Lane 1: No DNA control; Lane 2: uninjected embryos; Lane 3: morpholino-injected embryos. Embryos were injected with 200 μM tnnc1a, 200 μM tnnc1b, or 500 μM tnnc2 morpholino.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Dev. Dyn.