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Fig. S4 Specificity of tfap2a/e doubly-deficient melanophore defects. (A–F) Lateral views of live zebrafish embryos at 36 hpf. Insets show higher magnification of melanophores contained in the white boxes. (A–E) Sibling embryos injected with A), control MO, (C) tfap2e e2i2 MO, or (E) tfap2e AUG MO; all of these embryos exhibit normally pigmented melanophores. (B) A tfap2a mutant embryo injected with a control MO, with a reduction in melanophore numbers and melanophore migration, and slightly less than normal melanization. (D,F) tfap2a mutant embryos injected with (D) a tfap2e e2i2 MO or (F) a tfap2e AUG MO. These embryos display a further reduction in darkly pigmented melanophores, throughout the embryo. (G,H) Dorsal views of embryos at 36 hpf, anterior to the left. Embryos were first injected with tfap2a/e MO, followed by injection of mRNA encoding either (G) lacZ or (H) a dexamethasone-inducible version of tfap2a (tfap2aGR). Following injections, embryos were incubated in dexamethasone (Dex). The embryo injected with tfap2aGR shows rescue of pigmented melanophores whereas that injected with lacZ did not. Scale bars: 25 μM.