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Fig. S2 Standardization of SSC transplantation techniques. A. Transplantation device, in which a glass capillary needle is coupled to a peristaltic pump. B,C. Standardization of transplantation via using trypan blue to monitor the efficiency of the injections. Note trypan blue inside the testis (B) and ovaries (C). D,E. Genital pore histological sections stained with toluidine blue (male - D, female - E). Genital pore measurements: 120×80 µm dimensions, 63° angle (female); 75×50 μm dimensions, 60° angle (male). Anus (A), genital pore (G, arrow), and urethra (U). Scale bars = 100 µm. F. Ovary cell transplantation throughout injections into the lateral body wall (indicated by stippled line). G. Trypan blue was used to monitor the specificity of the injection. Note trypan blue inside the ovaries.