ZFIN Image: Erickson et al., 2010, Fig. S1

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Figure Caption

Fig. S1 Two independent meis1 morpholinos result in similar phenotypes. (A-D) Two independent meis1 translation blocking morpholinos effectively knockdown Meis1 protein, as shown by whole mount immunohistochemistry using a Meis1 monoclonal antibody. Hoechst 33258 stain marks the nuclei. (E-H) The meis1 non-overlapping (NOL) morpholino gives similar phenotypes as the ATG-morpholino (compare with Figure 5A-D and Figure 6F, G). meis1NOL morphants exhibit reduced foxd1 expression in the presumptive temporal retina (n = 27/29) (E, F), and upregulated fsta expression in the eye at 13 hpf (n = 19/19) (G, H). Dotted lines outline the optic vesicle. Views are dorsal with anterior to the left.

Acknowledgments

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