ZFIN Image: Goh et al., 2010, Fig. 1

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Figure Caption

Fig. 1 Rnd3 associates with Syx in mouse embryonic stem cells.

(A) Expression analysis of Rnds evaluated by RT-PCR from mES cells, mouse embryonic fibroblast (mEF) cells, embryoid bodies (EB) or plated differentiated mES cells (PD). (B) Identification of Rnd3-associated proteins. Rnd3 TAP-tagged proteins from transiently transfected mES cells using CBP-SBP vector (see methods). The purified proteins by coomassie staining is shown with identification by LC-MS analysis. (C) Expression levels of Rnd3 and Syx in NIE-115, HeLa and mES cells. Cell lysates were resolved by SDS-PAGE and immunoblotted with anti-Rnd3 and anti-Syx. (D) Endogenous localizations of Rnd3 and Syx in HeLa cells immuno-labeled with anti-Syx and Rnd3 antibodies. Rnd3 and Syx are both localized near the perinuclear region. Bar = 10μM.

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