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Figure Caption
Fig. 3 Dopamine induces aggregation of zebrafish parkin.
SH-SY5Y cells grown on glass coverslips were transiently transfected with FLAG-tagged zebrafish parkin. 24 h after tansfection, the cells were exposed to dopamine (5 mM, 5 h). The intracellular distribution of zebrafish parkin was analyzed by indirect immunofluorescence of permeabilized cells using an anti-FLAG monoclonal antibody (green). Nuclei were stained by DAPI (blue).
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