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Fig. 3 gup and sly are phenocopied by antisense morpholino oligonucleotides (MO). (A-D) Laminin 1 staining in 32 hpf (A) and 24 hpf (C) WT, and 32 hpf sly (B) and 24 hpf gup (D) mutant embryos. (E,F) Control MO (E) and sly/lamC1 MO (F) -injected embryos at 24 hpf stained with anti-laminin 1 antibody shows the loss of laminin 1 in sly/lamc1 morphants. (G-L) Embryos injected with gup/lamb1 MO (H,K) or sly/lamc1 MO (I,L) possess a shortened body axis, and show maintenance of ehh expression in undifferentiated notochords (K,L) when compared with control MO injected embryos (G,J).
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