ZFIN Image: Reynolds et al., 2010, Fig. 3

Image

Figure Caption

Fig. 3 Effect of overexpression of human VANGL1 variants on convergent extension in zebrafish embryos. Panels A–F show lateral views of 2 days post fertilization zebrafish. In Panel A, an uninjected wild-type fish is shown. In Panel B, a fish injected with high doses of VANGL1 alone show a severely reduced body length. Overexpression of either VANGL1^D259E (Panel C) or VANGL1^S467N (Panel D) or VANGL1^{M 328T} (Panel E) or VANGL1^V239I (Panel F) did not affect the body length in zebrafish embryos. In Panel G, VANGL1^R274Q RNA behaved like the wild-type VANGL1 (Panel B) and caused a severe reduction in body length when overexpressed. Panels H–L show dorsal views of 8–14 somites zebrafish embryos stained with MyoD. In Panel H, the converged and extended somites of an uninjected wild-type fish are shown. Overexpression of VANGL1 (Panel I) or VANGL^R274Q (Panel L) RNA caused wider somites lining the open neural tube, while overexpression of either VANGL1^M382T (Panel J) or VANGL1^V239I (Panel K) RNA did not affect this phenotype.

Acknowledgments

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Reprinted from Mechanisms of Development, 127(7-8), Reynolds, A., McDearmid, J.R., Lachance, S., Marco, P.D., Merello, E., Capra, V., Gros, P., Drapeau, P., and Kibar, Z., VANGL1 rare variants associated with neural tube defects affect convergent extension in zebrafish, 385-392, Copyright (2010) with permission from Elsevier. Full text @ Mech. Dev.