Fig. 1

Fig. 1 Etv5 functions as a positive effector in FGF signaling. (A–O) Lateral views and (P–T) animal views at shield stage. In situ probes and constructs injected are indicated on the left and above, respectively. Expression of downstream targets of FGF signaling, dusp6 and sef, are unaltered in etv5-injected embryos (B, G), but were induced by etv5:VP16 mRNA injections (C, H). Ectopic expression of dominant negative (etv5:EnR) blocks dusp6 and sef expression (D, I). Injection of etv5:T135D increased dusp6 and sef expression (compare A, F to E, J). Expression of bmp4 and chordin was unchanged in etv5-injected embryos (L, Q). A dorsalized phenotype was noted in etv5:VP16-injected embryos (M, R) and a ventralized phenotype upon etv5:EnR mRNA injection (N, S). Injection of etv5:T135D mRNA evoked dorsalized phenotypes (O, T). (U) Frequency of phenotypes elicited by microinjection of mRNAs indicated.