ZFIN Image: Stewart et al., 2010, Fig. 4

Image

Figure Caption

Fig. 4 Antiapoptotic Function of Shp2 Is Mediated by Its SH2 Domains

(A) Schematic of Shp2 mutants (also see Figure S1A).

(B) Acridine orange uptake in the dorsal neural tube of zebrafish embryos injected with the indicated mRNAs.

(C–L) Paired lateral views of 24 hpf embryos stained with Acridine orange; for each pair, the right panel shows a higher magnification image. (C) Control MO. (D) shp2 Ex3-MO causes cell death in the developing brain and spinal cord (arrow). Coexpression of WT shp2 (E), an LS mutant (A462T) (F), an A462T/R466M double mutant (G), or a truncation mutant that removes the PTP domain and C terminus (H), also blocks cell death. (I) WT embryos treated with U0126 do not mimic the morphant cell death phenotype. (J and K) Coexpression of shp2 mRNA with an N-SH2 point mutation (J) partially blocks cell death in the trunk; C-SH2 point mutations (K) fail to rescue. Coexpression of the C-SH2 domain alone rescues MO-induced cell death (L). Scale bars represent 200 μm. See Figures S1A and S3 for supporting data.

Error bars correspond to standard deviation of the mean.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Cell, 18(5), Stewart, R.A., Sanda, T., Widlund, H.R., Zhu, S., Swanson, K.D., Hurley, A.D., Bentires-Alj, M., Fisher, D.E., Kontaridis, M.I., Look, A.T., and Neel, B.G., Phosphatase-Dependent and -Independent Functions of Shp2 in Neural Crest Cells Underlie LEOPARD Syndrome Pathogenesis, 750-762, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell