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Fig. 1 Zebrafish tbx5 is required for pectoral fin initiation and outgrowth. All panels show the dorsal view of zebrafish embryos with anterior to the top. (A-E) Five dpf zebrafish embryos injected with control (A,C) or tbx5 (B,D,E) morpholinos. Embryos with 10 ng of tbx5 morpholino lack pectoral fins (B, asterisk) and Alcian Blue cartilage staining (E, asterisk), unlike control-injected embryos (A,C, arrows). Injection of a lower dose (2 ng, as in D) results in a milder fin phenotype such as a reduced pectoral fin blade (arrow in D).
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