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Fig. S2 Pharyngeal arch expression of jag1b and notch2 relative to dlx3b, dlx5a, hey1 and dlx2a. (A-H) Double-fluorescence in situ hybridizations at 36 hpf, with endodermal pouch outlines shown for reference in the merged panels. jag1b expression is largely non-overlapping with that of dlx3b and dlx5a. The stronger ventral expression of notch2 overlaps with dlx3b and dlx5a expression, with the border of dlx5a expression extending slightly more dorsally than that of strong notch2 expression. jag1b and hey1 are co-expressed in dorsal CNCCs, with arrows indicating where hey1 expression extends slightly more ventrally than jag1b. hey1 expression also overlaps with weaker dorsal notch2 expression in CNCCs. Colocalization of jag1b and notch2 with the CNCC-specific dlx2a probe confirms that jag1b is expressed in dorsal CNCCs and notch2 is expressed throughout arch CNCCs.