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Fig. S2 Sense control for the expression of sec24d in 3 dpf embryo by in situ hybridization shows no staining. Adherence Junctions Persist in bulldog Mutant Chondrocytes. (A–D) Pan-cadherin antibody predominantly labels N-cadherin in 3 dpf chondrocytes (green). Cadherin staining disappear as wild-type chondrocytes begin to lose cell-cell contacts (arrow in A,B). In contrast, bulldog chondrocytes (C,D) express uniform levels of Cadherin in all cells that is concentrated at cell-cell contact points (arrowheads). Nuclei are stained blue with TOPRO-3. Scale bars are 5 μM.
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