ZFIN Image: Carney et al., 2010, Fig. S4

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Figure Caption

Fig. S4 Confirmation of morphant phenotypes with second morpholino. Injection of second non-overlapping morpholinos was used to verify morphant phenotypes. Injection of translation-blocking morpholinos against fras1 (A), frem1a (B) and frem2b (D) into WT embryos realised blisters in the fin fold comparable to those seen with the original morpholinos. The fin blister phenotypes of rafels (C) and blasen (E) could be enhanced by the injection of morpholinos targeting the frem1b 5′UTR and frem3 ATG respectively. Co-injection of grip1 ATG and grip2 5′UTR morpholinos also yield strong blistering of the fin fold identical to that obtained with the original MOs (F).

Acknowledgments

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