Fig. 4

Fig. 4 Schematic of the genomic region from hoxa5a to hoxa3a with the tested elements in a color code (red: tissue-specific enhancer activity, blue: unspecific, white: no activity) and the distance of the element from the exons in kb. The expression of GFP under the regulatory control of the tested CNEs is shown in representative zebrafish larvae between stage 2 dpf to 4 dpf. The asterisk marks the level of the inner ear and the arrows follow a color code: red, hindbrain; yellow, spinal cord; brown, cranial ganglia; light blue, neuronal projection; pink, pectoral fin. a4-2. Expression of GFP in a dorsal and ventral subpopulation of cells in the most posterior part of the hindbrain and in the anterior spinal cord. a4-2′. Dorsal view of the posterior hindbrain and spinal cord with GFP expression in cells along the ventricular surface. a4-2″. Anti-GFP stained transgenic larva with sections trough the posterior hindbrain and anterior spinal cord (indicated by black lines). a4-3. Wide GFP expression in the medial posterior hindbrain and anterior spinal cord. a4-3′. Dorsal view that shows GFP expression in the vagal ganglion, in the pectoral fins and in fibers innervating the pectoral fins and the somites. a4-3″. Anti-GFP stained transgenic larva with sections showing signal in the hindbrain (red arrow points to the vagal nucleus) and in the spinal cord. The level of sections are indicated by the black lines. Neuronal projections are visible (1) from the vagal nuclei towards the vagal ganglion (brown arrow) and (2) into the pectoral fin and (3) along the somites.