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Fig. S3 Effects of the nkx6 knockdown on embryo general morphology and on the whole endoderm. A–D: bright-field pictures of control (A) nkx6.1 (B), nkx6.2 (C) and nkx6.1/nkx6.2 (D) morphants at 24 hpf. E–L: WISHs showing expression of foxa1 at 30 hpf (E–H) and myoD at 13 somites (I–L) in control (E; I), nkx6.1 (F; J), nkx6.2 (G; K) and nkx6.1/nkx6.2 (H; L) morphants; all views are ventral with anterior part to the left. Scale: 20 μm.
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Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.