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Fig. 2 Spatial and temporal expression pattern of tshz2 detected by RNA in situ hybridization. A-D: Embryos at 2 (A), 4 (B), 6 (C), and 8 (D) hours postfertilization (hpf). tshz2 mRNA is detected in early stages but signal fades by 8-10 hpf. Re-expression of tshz2 occurs around the prim-5 stage (24 hpf) (E,F). G,H: tshz2 expression pattern in a 36 hpf embryo. At 36 hpf, tshz2 is also expressed in the liver primordium (not shown). F,G: Dorsal views with anterior to the left. (E and H) Lateral views with dorsal up and anterior to the left. ba, branchial arches; f, pectoral fin; h, hindbrain; l, liver; op, olfactory placode; sc, spinal cord.