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Fig. 1 Whole-mount in situ hybridization analysis of zebrafish cldne. All embryos shown in lateral view, except (D) which is an animal pole view. A: Ubiquitous maternal expression of cldne at the eight-cell stage. B: Arrowhead indicates patchy cldne expression in the enveloping layer (EVL) at dome stage. C,D: Expression of cldne throughout the EVL at shield stage. E: Arrow indicates more intense hybridization in the EVL at the dorsal margin at 80% epiboly stage. Inset shows flat-mounted dorsal margin of an embryo stained for cldne (purple) and the dorsal forerunner marker cas (red). F: A 1 day postfertilization (dpf) embryo, in addition to faint cldne expression throughout the skin, cldne is detected in the olfactory placodes (arrow), ears (arrowhead), and proctodeum (asterisk).