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Fig. 5 Dla expression in the adult brain. Cross-sections of zebrafish adult brain labeled for Dla (red) and Elavl (green in A-C″), dla mRNA (D), or Dla (red) and glial fibrillary acidic protein (GFAP; green) in E-E″. B′,C′ show Dla and B″,C″ show Elavl labeling. A: Dla is widely expressed in 1-3 cell rows outlining the ventricle. Some Elavl-positive cells located near the ventricle do not express Dla. B-B″: Dla and Elavl protein labeling does not overlap. C-C″: Dla is expressed near the ventricle and in some scattered cells more distal to the ventricle (red arrows). Some Elavl-positive neurons differentiate close to the ventricle (green arrow). D: Consistent with Dla protein distribution, dla mRNA is expressed near the ventricle. E: Some GFAP-positive cells near the ventricle express Dla. E′ and E″ show the red or green channel respectively. F: Cartoon of an adult brain in a lateral view indicating the relative level of the cross section. v, ventricle. Scale bars = 125 μm in A, 50 μm in B-D, 7 μm in E-E″.