ZFIN Image: Yue et al., 2009, Fig. S9

Image

Figure Caption

Fig. S9 YY1-Binding Mutant Screening and Characterization
(A) Co-immunoprecipitation analyses using human β-arrestin1 truncations. The C-terminal region of β-arrestin1 showed strong interactions with YY1, while β-arrestin1 1-360 displayed least binding with YY1. (B) 7M β-arrestin1 interacts with known β-arrestin1 binding partners as shown by co-immunoprecipitation assay in HEK293 cells. (C) 7M β-arrestin1 showed normal ability of ERK activation. HEK293 cells stably expressing β2AR were incubated with 20 μM H-89 (a well known PKA inhibitor which blocks G protein mediated ERK activation) and stimulated with or without 1 μM isoproterenol (Iso) for 15 minutes. Equal amount of cell lysates were separated by SDS-PAGE and analyzed for pERK by western blot.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 139(3), Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G., Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression, 535-546, Copyright (2009) with permission from Elsevier. Full text @ Cell