Fig. 2 Identification of the cis-element required for the regulation by DAZL.
(A) Schematic representation of tdrd7 3′UTR mutant constructs. Putative DAZL-binding sites (GUUC; red, GUUA; yellow), miR-430 target sites (blue), and mutated DAZL-binding sites (gray) are indicated. Nucleotide positions relative to the stop codon are shown above. The exogenous miR-430 target site (IPTmiR-430) in the IPTmiR-430 91–123 and IPTmiR-430 91–123 mt constructs were derived from the nanos1 3′UTR [5]. (B) Injection experiments of mutant GFP-tdrd7 mRNA and DsRed mRNA with or without the mRNA encoding Myc-DAZL. GFP and DsRed were analyzed at 24 hpf. Fold change of normalized GFP fluorescence relative to mock control is shown on the right.