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Fig. 7 Induction of nos2a and nos2b gene expression. ZFL (A) or ZF4 (B) cell lines remained unstimulated (c) or were stimulated with LPS or PolyI:C for 8 or 20 h. Adult zebrafish (C) were challenged with LPS (5 μg in 50 μL) or PBS (as a negative control). Total RNA from cells or site of injection was reversed-transcribed and amplified to detect nos2a, nos2b, ifn1 and the β-actin control.
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Reprinted from Gene, 445(1-2), Lepiller, S., Franche, N., Solary, E., Chluba, J., and Laurens, V., Comparative analysis of zebrafish nos2a and nos2b genes, 58-65, Copyright (2009) with permission from Elsevier. Full text @ Gene