ZFIN Image: McMahon et al., 2009, Fig. 4

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Figure Caption

Fig. 4 Apoptosis in lmx1bdMO morphants is rescued by expression of lmx1b.1 cDNA. (A) Expression of GFP driven by the - 5 kb lmx1b.1 promoter. (B) Quantitation of the number of pyknotic cells present in ocular regions from wild-type, lmx1bdMO morphants expressing GFP, and lmx1bdMO embryos expressing lmx1b.1:ires:GFP. For each condition, n = 10 independent eyes were scored. Error bars represent Standard Error of the Mean. (C) Bright field image of lmx1bdMO morphants expressing GFP or (D) lmx1bdMO morphants expressing lmx1b.1:ires:GFP. (C′) Fluorescent image of panel C showing lmx1b:GFP-positive periocular cells (green) and pyknotic cells red). (D′) Fluorescent image of panel D showing lmx1b:lmx1b.1:ires:GFP-positive periocular cells (green) and pyknotic periocular cells (red).

Acknowledgments

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Reprinted from Developmental Biology, 332(2), McMahon, C., Gestri, G., Wilson, S.W., and Link, B.A., Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish, 287-298, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.