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Fig. S2 Initiation of ear collapse occurs around 3 dpf in lte mutants. Live DIC pictures of siblings (A,C,E) and to27d mutants (B,D,F) show that the ear sizes are comparable at 55 hpf (A, sib; B, mutant, identified by its ear collapse at 3 dpf) and that the semicircular canal projections are forming normally (black arrowheads). However, by 79 hpf, there is an obvious size reduction in the mutant (D) compared with the sibling (E), which is maintained at 4 dpf (E,H). asc, anterior semicircular canal; psc, posterior semicircular canal; usc, utriculosaccular chamber. Scale bar: 50 μm.
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