ZFIN Image: Lee et al., 2009, Fig. S2

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Figure Caption

Fig. S2 Transgenic activation of both Fgf and Ras induces Fgf target genes and wnt5b in embryos. (A) Whole-mount ISH of 30 hpf embryos from hsp70:dn-fgfr1, hsp70:ca-fgfr1, hsp70:dn-ras, or hsp70:v-ras transgenic lines that had been heat-shocked at 24 hpf. The expression of Fgf target genes pea3 and erm decreases in transgenic embryos with Fgfr or Ras inhibition, while their expression is increased and expanded in transgenic embryos in which Fgfr or Ras activity is boosted. Arrows indicate midbrain–hindbrain boundary. (B) Whole-mount ISH of 30 hpf embryos from wildtype and hsp70:ca-fgfr1 or hsp70:v-ras transgenic lines, that had been heat-shocked at 24 hpf. pea3 and wnt5b have similar expression domains in 30 hpf wildtype embryos (arrowheads), and expression of each is increased and expanded upon ectopic activation of Fgfr1 or Ras in transgenic embryos.

Acknowledgments

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Reprinted from Developmental Biology, 331(2), Lee, Y., Hami, D., De Val, S., Kagermeier-Schenk, B., Wills, A.A., Black, B.L., Weidinger, G., and Poss, K.D., Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins, 270-280, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.