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Fig. S1 Expression kinetics of apc, fgf8 and TGFP during development of apc mutants. Lateral view. Anterior to the left. (A–D) apc1 expression in wild-type embryos during development. At 24 hpf (A) and 30 hpf (B), apc1 is expressed in posterior telencephalon, cerebellum and ventral mid- and hindbrain. (C) At 36 hpf, apc1 expression expands dorsally into the tectum and anteriorly into the entire telencephalon. (D) At 48 hpf, apc1 is present almost ubiquitously throughout the brain. Inset in D — apc1 expression in apc mutant is strongly reduced, indicating non-sense mediated decay of mRNA. (E–H) Double WISH for fgf8 (blue) and TGFP (red). Insets — dorsal view. Dashed lines indicate the dorsal midbrain midline, MHB and cerebellum. (E, F) At 30 hpf, fgf8 expression in mutants expands into the domain ectopically expressing TGFP (arrowheads in F). (G, H) At 36 hpf, fgf8 partially overlaps with ectopic TGFP in the cerebellum (arrowhead and asterisk in H). ce, cerebellum; hin, hindbrain; MHB, mid/hindbrain boundary; ot, optic tectum; tel, telencephalon; vm, ventral midbrain. Scale bar 125 μm.
Reprinted from Developmental Biology, 331(2), Paridaen, J.T., Danesin, C., Elas, A.T., van de Water, S., Houart, C., and Zivkovic, D., Apc1 is required for maintenance of local brain organizers and dorsal midbrain survival, 101-112, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.