ZFIN Image: Aanstad et al., 2009, Fig. S7

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Figure Caption

Fig. S7 Morphological phenotypes in cilia knockdown embryos.
Injection of either dnKif3b mRNA (B-D), qilin MO1 (E-G) or qilin MO2 (H-J) gave similar morphological phenotypes in 24 hpf embryos. (A) shows an uninjected control embryo. 3 broad classes of phenotypes were found: class I embryos (B, E, H) resemble uninjected control embryos, class II embryos (C,F,I) have shortened body axes, and partial or full cyclopia, class III embryos (D, G, J) have severe gastrulation defects. The majority of embryos (>80% in all injections) in this latter class did not survive long after gastrulation (K) shows the percentage of embryos in each class for each injection experiment. Co-injection of qilin MO2 with qilin mRNA gave a significant rescue of the severity of phenotypes. The total number of embryos injected and counted for each injection is given in parenthesis, and is the result of a minimum of 3 independent experiments.

Acknowledgments

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