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Fig. 4 Cell proliferation was decreased in mats1 morphant embryos. (A-K) BrdU staining results at 24 hpf and tail bud stage. At the 24 hpf stage, BrdU levels were reduced in mats1 morphant embryos (D-F) compared to control wild-type embryos (A-C). While epiboly in wild-type embryos is finished at 10 hpf (G), this process is not completed in mats1 MO2 morphant embryos with the same age (J, K). Two to three more hours were needed for mats1 MO2 morphant embryos to reach tail bud stage (equivalent to 10 hpf of wild-type embryos at 28.5°C) (H, I). (L-O) PH3 antibody staining results. PH3 antibody staining results at tail bud stage and 24 hpf were consistent with BrdU staining, although the decreased degree of marked cell is not as distinctive as BrdU staining results. Anterior is towards left in all panels except for (J,K). Top view to see animal pole in (J), animal pole is towards top in (K).