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Fig. S3 olig2:EGFP+ axons and calretinin-immunoreactivity (CR-ir) in pretectal region. The brain of adult Tg(olig2:EGFP) fish were stained with anti-EGFP (green, A) and anti-calretinin antibodies (magenta, B). (C) Superimposed view of A and B. Sagittal section. The pretectal region is indicated by arrow. Note that both olig2:EGFP+ fibers and CR-ir+ neurons/fibers were detected in the preoptic regions, and also broadly in other regions of the brain. The abbreviations are described in Fig. 1. Scale bar: 500 μm.
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Reprinted from Developmental Biology, 330(2), Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M., Anatomy of zebrafish cerebellum and screen for mutations affecting its development, 406-426, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.