ZFIN Image: Park et al., 2009, Fig. S1

Image

Figure Caption

Fig. S1 Quantitative analyses. Representative semiquantitative RT-PCR results for the gfp (A), heart-specific markers vmhc and cmlc2 (B) in MO-injected embryos at 48 hpf. The assay used 1 μg of total RNA for the RT reaction. Control experiment was performed with 1 μg (1X) and 2 μg (2X) of total RNA (C). We consistently found significant decrease in expression levels of vmhc and gfp when comparing Mock and GATA4 and -6 double-deficient embryos (MO4+6). (D) The expression level of endogenous vmhc and cmlc2 mRNA after 48 h of injection of antisense morpholino nucleotides (MOs) against GATA4 and GATA6. Intensities of bands from three independent semi-quantitative RT-PCR experiments were measured (P < 0.05)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.