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Fig. 5 Developmental RT-PCR analysis of tbx22-1 and tbx22-2 mRNAs. A: The developmental expression of zebrafish tbx22-1 and tbx22-2 RT-PCR products, 402 and 515 bp, respectively, size fractionated in a non-denaturing TAE agarose gel. B: tbx22-1 and tbx22-2 RT-PCR products size fractionated in a denaturing, alkaline agarose gel. The non-denatured, heteroduplex, high molecular weight product present in A is eliminated in the alkaline denaturing gel. C: β-actin control RT-PCR products.
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