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Fig. 8 Tbx5 promotes β2 CaMK-II Expression. (a) β CaMK-II, δ CaMK-II and actin immunoblots of 10 μg NIH/3T3 lysate from cells which had been transfected with Tbx5-pCDNA3 or empty pCDNA3 vector. (b) CaMK-II specific activity (in pmol/min/mg) and integrated blot densities (normalized) from NIH/3T3 cells were averaged from four separate transfections and are shown with standard deviations. (c) Zebrafish embryos were injected with the indicated amount of pCDNA3 or Tbx5-pCDNA3 and activity assays performed at 72 hpf. Asterisks indicate P < 0.005.
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Reprinted from Developmental Biology, 330(1), Rothschild, S.C., Easley, C.A. 4th, Francescatto, L., Lister, J.A., Garrity, D.M., and Tombes, R.M., Tbx5-mediated expression of Ca(2+)/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin morphogenesis, 175-184, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.