ZFIN Image: North et al., 2009, Fig. 6

Image

Figure Caption

Fig. 6 The Effect of NO Signaling on HSC Development Is Cell Autonomous

(A) Cells from cmyb:GFP transgenic donor embryos, injected with nos1 ATG MO or control MO, were transplanted into lmo2:dsRed recipients at the blastula stage.

(B) Donor contribution to HSC formation assessed by confocal microscopy at 36 hpf. Shown are the merged picture on the top, red/green merge in the middle, and a high-magnification view of green fluorescence only on the bottom. cmyb:GFP donor-derived HSCs in recipients are highlighted by arrowheads.

(C) nos1 MO donors never contributed to HSC formation; the presence of cmyb:GFP-derived donor cells in the eye is indicative of a successful transplant.

(D) HSC chimerism in transplanted embryos (control versus nos1 MO, Fisher′s exact test, p = 0.0065, n ≥ 8).

Acknowledgments

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Reprinted from Cell, 137(4), North, T.E., Goessling, W., Peeters, M., Li, P., Ceol, C., Lord, A.M., Weber, G.J., Harris, J., Cutting, C.C., Huang, P., Dzierzak, E., and Zon, L.I., Hematopoietic stem cell development is dependent on blood flow, 736-748, Copyright (2009) with permission from Elsevier. Full text @ Cell