Fig. 8 Whole-mount in situ detection of Cx43.4 mRNA during late gastrulation and tail bud stages in wild-type and ntl mutant embryos. (A and B) Wild-type embryos at 90% and tail bud stage, respectively. (C and D) ntl mutant embryos at the same two stages, respectively. Dorsal views with animal pole or anterior to the left in all panels. Large arrows point to axial hypoblast showing labeled cells in wild-type embryos and an absence of Cx43.4 mRNA detection in ntl embryos, and small arrows in A and C mark the caudal embryonic shield. Scale bar in D represents 250 μm and is the same for the other panels.
Reprinted from Developmental Biology, 177(2), Essner, J.J., Laing, J.G., Beyer, E.C., Johnson, R.G., and Hackett, P.B. Jr., Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos, 449-462, Copyright (1996) with permission from Elsevier. Full text @ Dev. Biol.