ZFIN Image: Higashijima et al., 1997, Fig. 4

Image

Figure Caption

Fig. 4 Expression of GFP in transgenic embryos generated using α-actin–GFP. Embryos were viewed through their chorions, except for (B) where chorions were removed. (A) 30-h-old embryos of a B-rank transgenic line. Nonfluorescent embryos are nontransgenic siblings. (B) 28-h-old embryos of C-rank and D-rank transgenic lines. A nontransgenic embryo is also shown. (C) 26-h-old embryos of an A-rank transgenic line. (D) A 13-h-old embryo of an A-rank transgenic line. (E) A 26-h-old embryo of the most fluorescent line (A-rank). Film-exposure time for this figure was much shorter than that in other figures. Due to the high fluorescence of the muscles, other regions of the embryo are clearly visible.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 192, Higashijima, S., Okamoto, H., Ueno, N., Hotta, Y., and Eguchi, G., High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin, 289-299, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.