ZFIN Image: Stedman et al., 2009, Fig. S1

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Figure Caption

Fig. S1 Anti-Caspase 3 staining of apoptotic cells in Mo1bMo7 morphants. Control embryos (A) and Mo1bMo7Mop53 morphants (B–D) were stained with the otx2 and vhnf1 probes (purple staining) and with the anti-Caspase 3 antibody (brown staining). The black lines indicate the length of the anterior hindbrain, the region lying between the mhb and r5. Black arrows in A and B point to some of the Caspase 3 positive-cells. In the same experiment, some embryos were let to develop until the 5 s stage and were checked for krox20 expression in r3 as a control of the knock-down efficiency. In all of the embryos analysed (n = 10), krox20 expression in r3 was lost.

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Reprinted from Developmental Biology, 327(2), Stedman, A., Lecaudey, V., Havis, E., Anselme, I., Wassef, M., Gilardi-Hebenstreit, P., and Schneider-Maunoury, S., A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3, 566-577, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.