Fig. 3

Fig. 3 (A–O) Wild-type (A, F, K) and hnf1b¹⁸⁴³ (B–E, G–J, L–O) embryos were assayed for expression of krox20 (A–E), hoxa3 (F–J), and hoxb1a (K–O) by in situ hybridization. All panels are dorsal views of flat-mounted hindbrains with anterior to the top. Rhombomere numbering is indicated in panels (A), (F), and (K). (P) Wild-type (+/+), heterozygous (+/-), and homozygous hnf1b^hi2169 mutant (-/-) embryos were assayed for presence of hnf1b transcripts by RT-PCR. Virus integration site and locations of primers used for RT-PCR analysis are indicated in diagram at top. RT-PCR products were resolved by gel electrophoresis, and the resulting gel is shown at the bottom. (Q) Primer extension analysis of virus-hnf1b transcript. Top panel shows structure of wild-type hnf1b mRNA and indicates retroviral insertion site (triangle) in the hnf1b^hi2169 allele. Bottom panel shows putative viral-mRNA fusion transcript and indicates sequence obtained from primer extension analysis. (R–V) hnf1b^hi2169 embryos injected with an hnf1b spliceblocking morpholino (SPMO) show near-complete loss of krox20 expression in r5 compared to Figure 1B–E.