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Fig. S8 Reduction of pou47 expression in the anterior hypothalamus of embryos lacking Otp or Arnt2/Sim1 function. (A-D) Expression of pou47 at 48 hpf in wild-type (A), arnt2m1055 embryos (B), otpam866 embryos injected with 2 ng otpb morpholino (C) and otpam866 embryos co-injected with 2 ng otpb together with 1 ng sim1a morpholino (D). In embryos lacking Arnt2/Sim1 function (B), Otp function (C) or function of both Otp and Arnt2/Sim1 (D), the expression of pou47 in the preoptic area is strongly reduced compared with that in wild type (A, see arrowheads), whereas the expression in the ventral diencephalon corresponding to the area where ventral diencephalic dopaminergic groups are formed is only slightly reduced. (A-D) Lateral views. Anterior is towards the left. Scale bar: 50 μm. Abbreviations: d, diencephalon; H, hypothalamus; m, mesencephalon; PO, preoptic region; PT, posterior tuberculum; t, telencephalon.