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Fig. 7 Caffeine protects dopaminergic neurons from MPTP-induced neurotoxicity. Dorsal views of 5 dpf embryos hybridized with dat probe. Embryos were treated with drug from 24 hpf through 5 dpf. n = 30 embryos/treatment group. (A) Untreated control embryo. (B) Embryo treated with 40 μM MPTP. (C) Embryo treated with 10 μM caffeine and 40 μM MPTP. (D) Embryo treated with 10 μM caffeine. Neurons of the ventral diencephalon are circled. Black arrows point to the bilateral pretectal cluster of neurons.
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Reprinted from Gene expression patterns : GEP, 9(3), Boehmler, W., Petko, J., Woll, M., Frey, C., Thisse, B., Thisse, C., Canfield, V.A., and Levenson, R., Identification of zebrafish A2 adenosine receptors and expression in developing embryos, 144-151, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns