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Figure Caption
Fig. S2 myf5hu2022 mutation generates a likely null allele. (A) The genomic and protein structure of myf5 showing the nature of the hu2022 allele. (B) DNA sequencing traces from three individuals of each genotype. (C) Double in situ mRNA hybridization of myf5 (red) and the gene indicated (purple) in myf5+/hu2022 incross embryos at 13-15 ss. Dorsal flatmounts, anterior to top. myf5hu2022 mutants have low myf5 signal (arrowheads) compared with their siblings, but show no other change in mRNA expression.
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