ZFIN Image: Sittaramane et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Rescue of the tag1 morphant phenotype. Panels A–D show dorsal views of the hindbrain with anterior to the left. FBMN cell bodies and axons were visualized in Tg(isl1:gfp) embryos using anti-GFP antibody. Arrowheads indicate FBMNs. (A) FBMNs migrate normally in an uninjected control embryo. (B) FBMNs mostly fail to migrate in a tag1 morphant embryo. (C) FBMNs migrate normally in a tag1 mRNA injected embryo. (D) FBMN migration is mostly rescued in an embryo co-injected with tag1 MO and tag1 mRNA. (E) Quantification of the rescue data. The distribution of different phenotypes among the various treatments was analyzed by Pearson's Chi-Square statistics. The differences in the phenotypic distributions between the indicated pairs of samples were highly significant (*, P < 0.01; **, P < 0.001). Data from 4 experiments. Number in parenthesis denotes number of embryos. Scale bar in A (75 μm for A–D).

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Acknowledgments

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Reprinted from Developmental Biology, 325(2), Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A., The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish, 363-373, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.