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Fig. S2 Splice MOs recapitulate the phenotype resulting from injection of ATG MOs. In situ hybridization depicts expression of vmhc in (A) wild-type and (B) anti-nkx2.5 and anti-nkx2.7 splice MO-injected embryos. Embryos viewed dorsally, anterior to the top, at the 26 hpf stage. The vmhc-expressing portion of the nkx-deficient heart tube is substantially shorter and wider than the vmhc-expressing portion of the wild-type heart tube.
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Reprinted from Developmental Biology, 322(2), Targoff, K.L., Schell, T., and Yelon, D., Nkx genes regulate heart tube extension and exert differential effects on ventricular and atrial cell number, 314-321, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.