Fig. 5

Fig. 5 ogon and chordin encode partially overlapping functions. Crosses between ogo^m60/+;din^tt250/+ double heterozygotes resulted in five phenotypic classes: (A–E) WT, (F–J) ogo^m60/+;din1/+, (K–O) ogo^m60, (P–T) din, and (U–Y) double mutant. The caudal cell accumulation (arrowhead), duplicated tail fin (arrows), and ventral tail vesicle (open arrow) are characteristic of the mutant classes. In situ hybridization (18 hpf) using probes for krox20, expressed in hindbrain rhombomeres 3 and 5 (r3 and r5), and gata1, expressed in blood cells of the ventral tail, shows that the brain of (W) ogo^m60/ogo^m60;din/din double mutants is similar in size to that of (R) din homozygotes and that the increase in blood cells was slightly greater in (X,Y) the double mutant compared to either single mutant. krox20 expression is shown in dorsal views and gata1 expression is shown in dorsal and lateral tailbud views. Scale bars for live embryos, 200 and 50 μm for in situ hybridization preparations.