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Fig. 4 Loss of Tgfbi causes lateral detachment of muscle fibres. Live images of Tg(acta1:GFP)zf13 50-hpf control and morphant embryos. A: In wild type embryos, the muscle fibres are tightly connected to each other along their length. B: In tgfbi start site morphant embryos, by contrast, empty gaps were apparent in between muscle fibres. C: The same phenotype was observed following injection of a mixture of the start and splice morpholinos, each at half the concentration of that effective for single morpholinos.
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