ZFIN Image: Rai et al., 2008, Fig. S3

Image

Figure Caption

Fig. S3 Efficiency of morpholino knockdown of deaminase proteins and MBD4
(A-B) Morpholino knockdown efficiency of Apobec2a (4pg) (A) and Apobec2b (2pg) (B) was assessed at protein level by western blotting using whole cell extracts derived from wild type or morpholino-injected embryos. Anti-Apobec2a and anti-Apobec2b were specific rabbit antibodies generated from recombinant zebrafish proteins. Anti-Vinculin and anti-panH3 antibodies were used as loading controls. In panel B arrow indicates the band for Apobec2b whereas asterisk indicates a non-specific band. (C-D) Morpholino knockdown of AID and MBD4 was assessed at 80% epiboly in cDNA made from total RNA obtained from wild type embryos or embryos injected with AID Mo (4 pg) or MBD4 Mo (4pg). Spliced and unspliced DNA length is shown. 28S was used as a loading control.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 135(7), Rai, K., Huggins, I.J., James, S.R., Karpf, A.R., Jones, D.A., and Cairns, B.R., DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45, 1201-1212, Copyright (2008) with permission from Elsevier. Full text @ Cell