ZFIN Image: Gansner et al., 2008, Fig. 6

Image

Figure Caption

Fig. 6 Morpholino knockdown of col8a1 recapitulates the gul^m208 phenotype. A: Wild-type embryos injected with 12 ng of control morpholino exhibit normal morphology. B: Embryos injected with 2.4 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) characteristic of gul^m208 mutants. C: Reverse transcriptase-polymerase chain reaction (RT-PCR) at 24 hours postfertilization (hpf) demonstrating a dose-dependent reduction in col8a1 transcript after morpholino injection. D: Embryos injected with 12 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) that is worse than in gul^m208 mutants. Insets show notochord at higher magnification. All embryos were treated with PTU to inhibit melanin pigmentation and photographed at 30 hpf.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.