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Fig. 2 Spatiotemporal expression pattern of the xb51 gene in zebrafish embryos. Lateral views in A-C, E, G and J. Frontal views in D and F. Dorsal views in H and K. Anterior to the left (A-C, E, G, H, J, and K). A: At 20 hpf, xb51 transcripts were not detected in any embryonic region. B-F: xb51 expression was detected in forebrain from 22 hpf (arrow in B). At 24 hpf, the bilateral domains of xb51 expression were detected (C, D); its expression increased in the forebrain by 36 hpf (E, F). G-I: At 48 hpf, xb51 expression is extended to the diencephalon (G, H). In cross-section of the 48-hpf embryo, the diencephalic expression domains were present laterally close by pial surface (I, arrowheads). J-L: At later stages, xb51 expression domains extend to the midbrain, hindbrain, and retina (arrowheads). CCe, cerebellum; cm, craniofacial mesenchyme; dd, dorsal diencephalon; eth, ethmoid plate; dt, dorsal thalamus; h, hindbrain; Hy, hypothalamus; m, midbrain; mhb, midbrain-hindbrain boundary; OS, optic stalks; po, preoptic area; pq, palatoquadrate; Pr, pretectum; pt, posterior tuberculum; PTv, ventral part of the posterior tuberculum; ret, retina; s, subpallium; t, telencephalon; tc, tectum; tg, tegmentum; vd, ventral diencephalon; vt, ventral thalamus.