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Fig. S1 Quantitative PCR analysis of rbp4 mRNA in wild-type and els mutant zebrafish embryos. Amplification of the rbp4 gene (left panels) and a control gene, β-actin (right panels), from the wild-type and the els mutant cDNA pools. The amplifications by different PCR cycles from 22 to 28 are shown from top to bottom. There is no difference in the amplification of both genes from the wild-type and the els mutant cDNA pools, suggesting that the expression level of rbp4 mRNA is normal in the homozygous els mutant embryos.
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Reprinted from Mechanisms of Development, 125(11-12), Nishiwaki, Y., Komori, A., Sagara, H., Suzuki, E., Manabe, T., Hosoya, T., Nojima, Y., Wada, H., Tanaka, H., Okamoto, H., and Masai, I., Mutation of cGMP phosphodiesterase 6alpha'-subunit gene causes progressive degeneration of cone photoreceptors in zebrafish, 932-946, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.